Research Featured on The Cover

On the cover: Artistic rearrangement of leaf confocal micrographs displaying battery of fluorescently tagged cell fate determinants in response to injuries. Images created by Anju P. S., Dhanya Radhakrishnan and Abdul Kareem V. K. Artwork by Mabel Maria Mathew. See Research article by Radhakrishnan et al. (dev185710).

On the cover: The cover image depicts the expression of multiple cell fate determinants and the PLT2 transcription factor gradient in growing root tips. In this issue, Durgaprasad et al. report that dosage of PLT2, expressed in a gradient, orchestrates regeneration competence at the root tip. Image by Kavya Durgaprasad and artwork by Anju P S

On the cover: Artistic representation of the journey of de novo shoot regeneration in Arabidopsis. Merged confocal micrographs show the expression pattern of YFP-tagged PLETHORA (PLT) transcription factors (yellow) in leaf explant (bottom) and in regenerating progenitors and shoot primordia after inductive cues (top). In this issue, Kareem et al. (pages 1017–1030) report that PLT-mediated regulatory modules uncouple the acquisition of competence to regenerate shoot progenitors from completion of shoot regeneration, indicating a two-step mechanism for de novo shoot regeneration. Image by Abdul Kareem.

On the cover: Regenerating shoot from lateral root primordia in Arabidopsis. Merged confocal micrographs show the expression pattern of GFP tagged polar auxin efflux carrier PIN-FORMED1 (PIN1) membrane protein (green) and venus-YFP tagged auxin response sensor DR5 (yellow) in shoot primordia after inductive cues. In this issue, Kareem et al. (Regeneration 2016; 3(4):182–197) review the recent advances in understanding the molecular mechanism of de novo plant regeneration. Image by Abdul Kareem at IISER

On the cover: Santuari et al. reveal how the PLT gradient regulates cell state by region-specific induction of cell proliferation genes and repression of differentiation. The cover image shows how PLT2 expression influences the boundary of meristem target gene expression domains at the root tip. Activation of ProHAN:3xvYFP expression in a transgenic root after 0, 6, and 24 h of DEX induction of PLT2-GR expressed under control of the constitutive 35S promoter. Ectopic induction is obvious at 6 and 24 h of transfer to DEX media from the enhanced expression of fluorescent protein throughout the root outside of the meristematic domain of endogenous PLT2 expression. This image series shows that PLT levels determine the proximal/shootward boundary of the target gene expression domain, consistent with a dose-dependent role in zonation.

Research in News

How wounded plants heal survive
The Hindu, March 07, 2020

Decoding how plant roots regenerate
The Hindu, October 19, 2019

Top journal hails research on plant regeneration
Deccan Chronicle, November 18, 2015

City Scientists Unravel Mystery of Plant Regeneration
The New Indian Express, November 17, 2015

Video by the Hindu on our work

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Recent Publications

● Shanmukhan AP, Mathew MM, Aiyaz M, Varaparambathu V, Kareem A, Radhakrishnan D, Prasad K*, (2021) Regulation of touch-stimulated de novo root regeneration from Arabidopsis leaves, Plant Physiology, 187 (1), 52-58. (Selected as Plant Physiology Article of the Week by American Society of Plant Biologists (ASPB))

● Radhakrishnan D, Shanmukhan AP, Kareem A, Aiyaz M, Varapparambathu V, Toms A, Kerstens M, Valsakumar D, Landge AN, Shaji A, Mathew MK, Sawchuk MG, Scarpella E, Krizek BA, Efroni I, Mähönen AP, Willemsen V, Scheres B, Prasad K*. (2020) A coherent feed-forward loop drives vascular regeneration in damaged aerial organs of plants growing in a normal developmental context. Development. 147(6):dev185710. doi:10.1242/dev.185710 (Selected for highlights of the issue and Featured on Cover)

● Shanmukhan AP, Mathew MM, Radhakrishnan D, Aiyaz M, Prasad K*. (2020) Regrowing the damaged or lost body parts. Current Opinion in Plant Biology. 53:117‐127. doi:10.1016/j.pbi.2019.12.007

● Durgaprasad K, Roy MV, Venugopal MA, Kareem A, Raj K, Willemsen V, Mähönen AP, Scheres B, Prasad K*. Gradient Expression of Transcription Factor Imposes a Boundary on Organ Regeneration Potential in Plants.
Cell Reports. 2019;29(2):453‐463.e3. doi:10.1016/j.celrep.2019.08.099 (Recommended by F1000 and Featured on Cover)

● Radhakrishnan D, Kareem A, Durgaprasad K, Sreeraj E, Sugimoto K, Prasad K*. (2018) Shoot regeneration: a journey from acquisition of competence to completion. Current Opinion Plant Biology. 41:23‐31. doi:10.1016/j.pbi.2017.08.001

● Kareem A, Durgaprasad K, Sugimoto K, Du Y, Pulianmackal AJ, Trivedi ZB, Abhayadev PV, Pinon V, Meyerowitz EM, Scheres B, Prasad K*. (2015) PLETHORA Genes Control Regeneration by a Two-Step Mechanism. Current Biology. 25(8):1017‐1030. doi:10.1016/j.cub.2015.02.022 (Featured on Cover)

● Santuari L, Sanchez-Perez GF, Luijten M, Rutjens B, Terpstra I, Berke L, Gorte M, Prasad K, Bao D, Timmermans-Hereijgers JLPM, Maeo K, Nakamura K, Shimotohno A, Pencik A, Novak O, Ljung K, Heesch SV, de Bruijn E, Cuppen E, Willemsen V, Mähönen AP, Lukowitz W, Snel B, de Ridder D, Scheres B, Heidstra R (2016) The PLETHORA Gene Regulatory Network Guides Growth and Cell Differentiation in Arabidopsis Roots. Plant Cell. 28(12):2937‐2951. doi:10.1105/tpc.16.00656 (Featured on Cover)

● Mähönen AP, Ten Tusscher K, Siligato R, Smetana O, Díaz-Triviño S, Salojärvi J, Wachsman G, Prasad K, Heidstra R, Scheres B. (2014) PLETHORA gradient formation mechanism separates auxin responses. Nature. 515(7525):125‐129. doi:10.1038/nature13663


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